OBJECTIVE To observe the effects of
Ganzhifu mixture on the course of experimental
hepatic fibrosis and to explore the mechanism.METHODS
Forty five Wistar rats were used in this study comprising four groups: normal group,Ganzhifu group,colchicine group and model group.Except normal group,the other three groups were given subcutaneous injection of CCL_4 and drinking 10% alcohol so as to make the model of
hepatic fibrosis.At the same time the rats of Ganzhifu group were given the herbal remedies via gastric cannel once each day,the rats of colchicine group were given colchicine by same way,the rats of model group were given normal saline.At the end of the experiment,liver function,malondialdehyde(MDA) content,superoxide disumutase(SOD) activity of liver tissue were measured,respectively.Tissue microarray sections of liver were stained with HE,Masson and Gorden-Sweet to observe pathological changes and calculate the point according to inflammation and fibrosis semiquantative scoring system(SSS) of liver tissue.RESULTS Compared with model group,liver function was improved remarkably MDA content changed from 16.31±6.03 nmol·mg~(-1) to 8.84±2.08 nmol·mg~(-1)(P<0.01)accompanying with a striking rise of SOD activity from 86.33±17.74 U·mg~(-1) to 122.01±19.12 U·mg~(-1)(P<0.01).Pathological findings showed that the Ganzhifu group had less inflammation and lower degree of fibrosis.The effect of Ganzhifu on liver fibrosis was parallel with colchicine.CONCLUSION Ganzhifu can protect liver and prevent or alleviate the rats hepatic fibrosis.The mechanism of Ganzhifu is antioxidation possibly.