Objective To investigate the protective effects of
carbachol on organ
dysfunction induced by gut
ischemia-reperfusion and on the synthesis and expression of inflammatory cytokines in the
intestinal tissue and the
leukocytes.Methods Models of superior mesenteric artery obstruction (SMAO) in rats and superior mesenteric artery partial obstruction (SMAPO) in rabbits were adopted. The indicators of organs function, mRNA expression of cytokines in leukocytes and TNFα contents in the intestinal tissue and the blood were detected at different time points.Results In SMAO group, the plasma ALT, BUN and CK of rats treated with carbachol were lower than those in rats without carbchol at 2 hours after reperfusion; the mRNA expression of TNFα in leukocytes was down-regulated and those of IL-4, IL-10 and IFN were up-regulated in rats treated with carbachol.In SMAPO group, ALT, Cr and CK-MB in the plasma increased during
Ischemia and at early reperfusion stages in rabbits treated with carbachol, but the enhanced extent was less than those without carbachol, and returned to the normal during 1 to 3 days after reperfusion; the content of TNFα in the intestinal tissues of rats with carbachol was less than that without carbachol.Conclusion Carbachol could improve liver, kidney and heart dysfunction,inhibit the synthesis of TNFα in the intestinal tissues, and regulate the expressive proportion of pro- and anti- inflammatory cytokines.
More abstracts about the Protective effects of carbachol on organ dysfunction induced by gut ischemia-reperfusion in animals