Objective To study the expressions of MARCKS and p-MARCKS in the
hippocampal cortex in the rats with multiple acute cerebral
infarct, observe the effects of 9601-Granules on MARCKS signal transduction pathway, and investigate the protective mechanism of TCM drugs against acute cerebral ischemia on the basis of proteins. Methods The
rat model of multiple acute cerebral infarct was established by using modified Kaneko method; changes in the scoring of symptoms and signs of nervous system, and changes in the pathology and cell ultrastructure of hippocampal cortex, were observed in each experimental group; changes in the
expressions of MARCKS and p-MARCKS in hippocampal cortex experienced acute ischemia and the regulating effects of TCM drugs on their expressions were detected by using immunohistochemical method and Western blot method. Results The levels of the expressions of MARCKS and p-MARCKS in hippocampal cortex were higher in the group experienced acute ischemia than those in the normal control group (P<0.05). Although both 9601-Granules and Nimodipine could decrease the abnormal elevation in the expressions of MARCKS and p-MARCKS, there was no significant difference between the effects of the two drugs. Conclusion Under the condition of acute cerebral ischemia, the expressions of MARCKS and p-MARCKS are abnormally increased in hippocampal cortex in the rats, which is closely related with ischemic defect; and 9601-Granules possess marked downregulating effects on the abnormal increase.