Objective To investigate the influence of astrocyte-conditioned medium (ACM), ACM treated with Kangdai-1, and ACM plus
Kangdai-1 on the neurons damaged by simulated
cerebral ischemia/reperfusion in vitro.Method First, the isolation, purification and cultivation of rat cortical astrocytes and neurons, and establishment of the model of stimulated cerebral damage by ischemia/reperfusion were carried out. Then, the ACM, ACM treated with Kangdai-1, and ACM plus Kangdai-1 collected 18 hours after ischemia/reperfusion, were used to culture the damaged neurons in a concentration of 1∶5. Last, the activity, survival rate and death rate of the cultured neurons, the culture fluid leakage rate of LDH, and the expressed amount of NOS-intense positive cells, were detected. Results All ACM, ACM treated with Kangdai-1, and ACM plus Kangdai-1 could markedly increase the activity and survival rate of the damaged neurons, and markedly decrease the death rate of the damaged neurons, the culture fluid leakage rate of LDH, and the expression of NOS-intense positive cells, with a sequence of potency as: ACM treated with Kangdai-1>ACM plus Kangdai-1>ACM. Conclusion (1) The neurons damaged by simulated cerebral ischemia/reperfusion in vitro showe a phasic change of damage-compensation-redamage-restoration; (2) ACM showes significant protective and repairing effects on damaged neurons; (3) Astrocytes play an important role in the process of
brain ischemic preconditioning; (4) Kandai-1 may indirectly protect and repair damaged neurons through astrocytes.