Objective To observe the long-term drug toxicity of Lianhudan burn plaster after continue using on local skin of rabbit
for 13 weeks. Methods Thirty-six
rabbits were selected with half male and half female, and divided into 3 groups according to body weight randomly:the control group with normal skin, the group with low dose (5g/every rabbit, per day) of Lianhudan burn plaster and the group with high dose(15g/every rabbit.day). The were used for 7 days every week and continue to 13 weeks. The change of appearance and behave of rabbits should be observed every day during experiment term. The blood was taken from periotic artery of rabbit respectively at 13 weeks and 2 weeks after drug stopping. The blood constituent analysis (RBC, HB, WBC Classification and PLT) and serological biochemical
examination (AST, ALT, ALP, BUN, Crea, TP, ALB, TBIL, TC and GLU) were proceeded. The bodies of animals were dissected and examined.Their organs was weighted,then the organ coefficient was calcu-lated. The skin were taken from where drug using and proceeded pathological examination.Results After given the plaster with low and high dose for 13 weeks, the rabbits with normal skin or injuried skin have good condition: no variation causing by the drug in appearance, behavior,body weight, blood examination, blood biochemical analysis and histological pathological examination comparing with the control group. Only, the rabbits with skin defects also. Cornification of stratified pavement epithelium and widen of hair follicle can be seen on skin site used the drug by pathological examination. The site was caused mainly by drawing injury. recovering to normal condition later two weeks after drug stopping.Conclusion It is safe to apply Lianhudan burn plaster in clinic, but without long-term drug toxicity.<