Aim:To study the effect of PF4 gene transfected
attenuated Salmonella Typhmuriumas orally on the hematopoiesis reconstitution
of mice after large dose of
chemotherapy. Methods: Mice were treated with PIRES2-EGFP/ PF4
transfected attenuated Salmonella Typhmuriumas before and after large dose chemotherapy. Survival rate of mice after chemotherapy, the number of bone marrow cells,-Sca-1 and C-kit cells and colony forming units of different lineages in various time were detected. Results: PIRES2-EGFP/PF4 transfected attenuated Salmonella Typhmuriumas treated mice both before and after chemotherapy showed increased survival rate than those only treated with before chemotherapy. Both groups treated with PIRES2-EGFP/PF4 transfected attenuated Salmonella Typhmuriumas showed greater survival rate than the PBS group and non-carrier attenuated Salmonella Typhmuriumas group. Between the 9th and the 12th day after chemotherapy, in PIRES2-EGFP/PF4 transfected attenuated Salmonella Typhmuriumas group, platelet in peripheral blood and the percentage of Sca-1+ , C-kit+ cells were greater than in the control group. Bone marrow cells and their mix-CFU showed remarkable increase in the 5th ,9th and 12th day. Conclusions: Oral attenuated Salmonella Typhmuriumas SL3261 transfected with PF4 gene can protect mice from injury and promote the hematopoiesis reconstitution of chemotherapy injured mice.