Objective To study the effect of chinese medicine SHUANGJIAWULING on the expression of Ⅰ, Ⅲ collage using immune induced
liver fibrosis rat model.Methods Rat liver fibrosis was induced by the administration of 200g/L human serum albumin (HSA) by iv.Ninety rats were divided into 6 groups. Group A is as untreated control.In group B,chinese medicine SHUANGJIAWULING was taken orally at the same time with the administration of HSA. In group C,Chinese medicine SHUANGJIAWULING was taken orally immediately after the administration of HSA. In group D,chinese medicine SHUANGJIAWULING was taken orally at 3 months after the administration of HSA. In group E,colchine tablets was taken orally at the same time with the administration of HAS. In group F,chinese medicine BIEJIARUANGANPIAN was taken orally immediately after the administration of HSA.In group G, HSA was administrated without treatment.The pathological changes of liver tissue were observed by HE and Von-Gieson staining. The mRNA and proteins of Ⅰ, Ⅲ collage in the liver were analyzed by in situ hybridization and immunohistochemistry, respectively. Results In the immune induced liver fibrosis model rats, the fibrosis was most serious at 3 months after the administration of HAS. The expression of Ⅰ, Ⅲ collagen was the strongest at the same time. The liver fibrosis and expression of Ⅰ, Ⅲ collagen were reduced significantly in the SHUANGJlAWULING treatment rats (P<0. 05). In the SHUANGJIAWULING treatment rats, group B is better than group C for the efficacy. SHUANGJlAWULING treatment was superior to colchine tablets treatment.Conclusion Chinese medicine SHUANGJIAWULING can reduce liver fibrosis by inhibiting the expression of TIMPs expression and increase active-MMPs, then the degradation of the extracellular matrix is accelerated of Ⅰ, Ⅲ collagen mRNA and protein expression.