BACKGROUND:If it can prove the therapeutic effects of acidic peptide when appl ying it to the mouse model of vascular dementia(VD). OBJECTIVE:To study the memory ability,change of contents of protein and nitric oxide of mouse model of VD and make comparison with normal mice. DESIGN:A randomized and experimental study. SETTING:Biochemistry &Molecular Biology Teaching Section of College of Medici ne,Zhengzhou University. PARTICIPANTS:The experiment was completed in the First Research Unit and Anima l Centre of Biochemistry &Molecular Biology Teaching Section of Zhengzhou Unive rsity.Totally 84 mice of Kunming species were selected and the mouse was exclude d because its average response time was slower than normal mouse when performing step down avoidance test.The mice were randomly divided into 7 groups:normal g roup,model group,saline group,naofukang group,high dose acidic peptide group,mod erate dose acidic peptide group and low dose acidic peptide group. METHODS:All the mice excepts of 12 in normal group were conducted stomach perf usion of high fat emulsion 0.5 mL per day for 10 days to establish VD animal mod el according to the method of Gao et al.There was no treatment for model group. While saline, acidic peptide high,moderate and low dose of acidic peptide was or ally administrated to saline group,naofukang group,high dose,moderate dose and l ow dose acidic peptide group respectively for 15 days.Then learning and memory f unctions were tested by step down avoidance test.After being executed, protein and nitric oxide in mouse brain was assayed.
MAIN OUTCOME MEASURES:Learning and memory ability of mice in each group as wel l as the content of protein and nitric oxide in brain. RESULTS:①The result of step down avoidance test showed that acidic peptide h ad greatly reduced the mistaken times in mice and dramatically improved the lear ning and memory ability of dementia mice.②The biochemical analysis showed that the content of protein in mice of model group <(3.530 9±0.327 8) g/L>was redu ce d comparing with mice in normal group <(4.028 3±0.378 3) g/L>. After administ ra ting acidic peptide to dementia mice,the protein content in high,moderate and lo w dose group was(4.908 3±0.515 3) g/L,(4.752 5±0.471 7) g/L and(4.376 7±0.370 7) g/L respectively which was greatly increased when compared with model group and saline group. It suggested that acidic peptide could stimulate the synthesis of protein in brain,which was good for the repair of brain cell and its functio ns.③acidic peptide greatly inhibited the synthesis of nitric oxide and reduced the toxic effects of NO to brain cells. CONCLUSION:Acidic peptide can improve the ability of learning and memory of mi ce with VD greatly and also has good effects on the plerosis of injured cerebral cells and the rehabilitation of its function.