Aim To observe the damages induced by h ydrogen
peroxide in
cultured bovine cerebral microvascular endothelial cells (BC MEC) and evaluate the protective effects of
hydroxyethylpuerarin on
hydrogen per oxide-injured BCMEC. Methods BCMEC were cultured and transferred into modified Eagle medium (MEM). The viability of cells was detected by MTT assay. Cell
injury was determined by lactate dehydrogenase (LDH) activity in the extracellular medium. Flow cytometry was employed to observe the occurrence of apoptosis. Morphologic changes of cells were visualized under phase contrast and electron microscopes. Results Hydrogen peroxide (200 μmol·L -1 for 4 hour s) inhibited the viability of cultured BCMEC and stimulated
LDH release. Hydroge n peroxide (100 μmol·L -1 for 4 hours) induced the occurrence of apo ptosis. Hydroxyethylpuerarin was shown to increase the survival rate and decreas e the activity of LDH of BCMEC damaged by hydrogen peroxide. Hydroxyethylpuerari n was also found to protect BCMEC against
apoptosis induced by hydrogen peroxide . Conclusion Hydrogen peroxide induces BCMEC injury either by apo ptosis or through necrosis. Hydroxyethylpuerarin protects BCMEC against hydrogen peroxide-induced injury in a concentration-dependent manner. Its antioxidant effects might be involved as the mechanism protection.
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