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Shvoong Home>Medicine & Health>Effect of ginsenoside Rb1 on the expression of GLUT1 in penumbra area after cerebral ischemia/reperf Review

Effect of ginsenoside Rb1 on the expression of GLUT1 in penumbra area after cerebral ischemia/reperf

Article Review   by:TsingHua     Original Author: Chinese Journal of Experimental Surgery
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Objective To investigate the change of infarct size and the expression of GLUT1 in penumbra area after cerebral ischemia/reperfusion injury in rats and the effect of ginsenoside Rb1 on them.Methods Forty-two male adult SD rats were randomly divided into 3 groups:sham-operated group,the control group and the ginsenoside Rb1 group.The model of middle artery occlusion (MCAO) in rats was established by using an intraluminal thread technique.The influence of ginsenoside Rb1 on infarct size,neurologic deficit,the expression level of GLUT1 mRNA and protein in the penumbra were observed.The infarct size was analyzed quantitatively by Kontron IBAS 2.5 image auto-analyses system.The change of GLUT1 mRNA was assessed by RT-PCR.The expression of GLUT1 protein was detected by immunohistochemistry.Results The volume percentage of infarct in sham-operated group,the control group and the ginsenoside Rb1 group was 0, (0.04± 0.01) % and (0.02± 0.01) % respectively (P<0.01).The level of GLUT1 mRNA in sham-operated group,the control group and the ginsenoside Rb1 group was 0.23± 0.14, 0.55± 0.04 and 0.79± 0.19 (P<0.05) and the PU value of GLUT1 protein expression was 5.1± 1.2, 13.1± 1.7 and 19.0± 1.9 respectively (P< 0.05).The GLUT1 mRNA and protein levels in ginsenoside Rb1 group were much higher than in the control group.Conclusion Ginsenoside Rb1 could decrease the infarct size after cerebral ischemia/reperfusion injury.Rb1 maintained the energy supply of the brain via upregulating GLUT1 expression,which may be one of the protective mechanisms.
Published: February 28, 2005   
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