To study the effects of Ginaton (Ginkgo biloba extract) in prevention of liver fibrosis in mice. The experimental liver fibrosis induced by tetraachloromethone in rats was treated with Ginaton (Ginkgo biloba extract). Wistar rats were divided into three groups: Ginaton group (n =8); CCl4 group (n =8); control group (n =8). Ginaton group and CCl4 group mice were induced by 40% CCl4 olive oil solution into models of liver fibrosis, Ginaton group was injected the Ginaton solution in abdomino (8 ml/kg). Liveer histological changes; hydroxyproline (HYP)and maloldialdehyde (MDA) content in liver tissues; senum PC-Ⅲ, HA, TXB2 and 6-keto-PGF1a levels were observed. The pathlogical studies showed that the liver fibrosis degree of Ginaton group was lighter than that of CCl4 group. Ginaton group has significantly lower level of HYP (166±27 μg/g), MDA (206.3±1.2 umol/g), PC-Ⅲ (113.0±42.0 μg/L) and HA (258.0±92.0 μg/L) than those of CCl4 group (HYP 248±45 μg/g, MDA 273.5±47.6 μg/g, PC-Ⅲ 256.0±68.0 μg/L and HA 479.0±116.0 μg/L, respectively); Ginaton group TXB2 reduce (78.44±32.8 ng/L), CCl4 group (94.5±48.4 ng/L) and 6-keto-PGFla increase (15.1±5.4 ng/L, CCl4 group 12.45±5.48 ng/L). [Conclusion] Ginaton has protection of hepatic cells hepatocellular death and can prevent the formation of liver fibrosis.