Objective: Proteomics is currently in a phase of technological development and establishment, demonstrating the capacity
for high throughput, is a major challenge. It is used in life science research, particulay in the mechanism of tumrogenesis.
Yinchenwulin powder, which was extracted from Chinese herbs, is used to treat the patients with atherosclerosis. We investigated the molecular mechanism of Yinchenwulin Powder on the atherosclerotic rats in this paper. Methods: The AS model wistar rats made by feeding with high grease food and AS enplaques were observed in the aorta after one month of feeding. By
two-dimension electrophoresis,the proteins were separated, and as well as protein profiles of aorta tissue. Compared with the protein profiles of aorta tissue treated, model proteins in different expression were identified by Image-Master ID Elite 4.01 .Result: There were 1077+68 protein dots in aorta tissue treated,and 1106+84 in aorta tissue. Compared the protein profiles of aorta tissue treated with model, 556 proteins were found as dys-regulated. Among them,196 proteins were observed up-regulated and 364 proteins were seen down-regulated in aorta tissue treated.Conclusion: Yinchenwulin Powder which could treat atherosclerotic rat may associated with those protein regulated by itself,and it will provide a new method of molecular mechanism of Yinchenwulin Powder.