AIM: To investigate the
protective effect of Alarelin on cisplatin-induced
ovarian toxicity in female rats.METHODS: Forty
12-week-old female Wistar rats were randomly divided into four groups.Group A received cisplatin (2 mg/kg Qd×10) intraperitoneally,Group B received Alarelin (5 μg Qd×10) subcutaneously before the administration of cisplatin (2 mg/kg Qd×10),Group C received only Alarelin (5 μg Qd×20 ) subcutaneously,and Group D,used as normal controls,received intraperitoneal administration of 9 g/L sodium chloride.Blood samples were collected from the heart and the levels of blood follicle stimulating hormone (FSH) and estradiol (E 2) were determined.Both ovaries and uterus of each rat were removed to measure the amount and the maturity of follicles.Body mass and histomorphological features of the rats were also observed.RESULTS: Compared with that in control group,the mass of ovary and uterus decreased (P<0.0001) and a significant reduction was observed in the number of ovarian follicles at each grade (P<0.0001).The level of E 2 significantly lowered (P<0.0001) and the levels of FSH markedly ascended in Group A.Compared with that in Group A,the amount of primitive follicles significantly increased in group B (P<0.0001) and Alarelin showed markedly
protective effect on the ovarian and uterine morphological construction of rats.CONCLUSION: Alarelin can improve the histomorphologic features of ovary and uterus in pathologic rats.Alarelin has some protective effect on cisplatin-induced
ovarian toxicity in rats.