Aim The goal of this research project is to study if t opically applied with high concentration
Mitomycin C (MMC) or in
contacted with it long time will help the healing of injured nasal mucosa and to observe the pa thomorphology of the nasal mucosa in rall.Methods Same treatmen ts were carried out on nasal mucosa of 40 rabbits.Injured rabbits were randomly divided into four groups,including normal saline control group(A),MMC at conce ntration of 0.4mg·ml -1、used 5 minutes(B),0.4mg·ml -1、used long ti me(C),and 1.0mg·ml -1,used five minutes(D).For each rabbit,an absorbable gelatin sponge soaked with medicine applied to the site of injured nasal mucosa for corresponding time.Pathomorphology of different groups were
observed and det ected with light and electronic microscopes after 1 month of the corresponding t reatment.Results The rate of the adherence in B groups was sign ificantly different from that in group A (P<0.05).There was no adherence for mation in group C and D.But there were 6 cases of perforation of nasal septum in group C and 2 cases in group D.Under light microscope and electronic microscope ,there is an antiproliferative effect on fibroblasts in B、C、D groups and in gr oup B nasal mucosal healing is well.We observed many necrosis cells in group C a nd D,we also observed the injured mucosa is discontinuation in group C and part of cilias appeared abnormal structure in group D.Conclusions M MC was found to be efficient to inhibit fibroblast proliferation during this res earch project,but high concentration or long time applied with MMC at the injure d nasal mucosa have no good to the healing of it.MMC at concentration 0.4mg·ml -1 used 5 minutes was safe to use.