Objective To investigate the protective effect of
Stronger Neo-Minophagen C (SNMC) on Fulminant
Liver Failure (FLF) and it's possible mechanisms. Methods D-GalN and lipopolysaccharide (LPS) were injected into the abdominal cavity of rats one time to establish experimental model of FLF and the mice were protected by SNMC. The survival rate of mice, the level of liver function, inflammatory factors and liver histopathology of each group were observed. The structure of mitochondrion was detected by electron-microscope. The hepatocyte
apoptosis in-situ was evaluated by TUNEL.
cyt-C and
caspase-3 which were determined by immunohistochemical method. Results D-Gal N and LPS can be used to establish model of FLF. There was an obvious decline of plasma TNF-α, NO, ET-1, IL-6. Meanwhile, the damage of hepatic tissues in SNMC-treated groups by different doses (large
dose, medium dose, small dose) and different time point (before, during and after administration of SNMC)compared with that of control group (P < 0.01).It indicates that SNMC can be used to treat FLF. There is no difference among groups given different doses and different time (P>0.05). Nevertheless, there is significant difference of survival rate between SNMC-treated groups and the control group. The apoptosis index was 32.3% at sixth hour after giving SNMC and decreased to 5% at seventh day after giving SNMC in C group. In the meantime, the quantity expression of Cyt-C and caspase-3 in A group decreased with the time and the prototypic hepatocyte apoptosis was found to be improved by electron-microscope with time in treatment group. Conclusions SNMC can effectively protect the
Liver damage of FHF mice. The mechanism may be involved in the inflammation reaction induced by all kinds of inflammatory factors and SNMC can inhibit the release of Cyt-C as well as the activation of caspase-3 by stabilizing the membrane of mitochondrion, thereafter the apoptotic progress of hepatocytes were prevented.
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