Objective To investigate the
antitrichomonas effect of propolis from Henan Province of China on
Trichomonas vaginalis after
being cultured for different hours in vitro. Methods The culture of T. vaginalis were divided into three groups: the propolis group, the solvent group and the control group. The concentration of propolis in the medium of propolis group was 2.4 mg/ml, equivalent amount of solvent was added to the solvent group, and the control group was normally cultured. The number of the parasite, the number of the survivals and the survival rate at 0, 6, 12 and 24 hours were observed and recorded respectively after being cultured. Results After being cultured for 6, 12 and 24 hours, the number of the survivals(10~4 /ml) in the propolis group was: 7.04±1.75, 2.87±0.69, 1.24±0.59; the survival rate (%) was: 43.00±6.83, 22.25±5.32, 11.50±5.74. In the control group, the number of the survivals: 36.77±12.28, 50.09±17.19, 79.61±22.09; the survival rate: 94.50±3.51, 90.00±4.32, 97.25±0.96 respectively. Significant difference was found between the propolis group and the others. Conclusion Propolis collected from Henan Province possesses obvious
antitrichomonas activity in vitro, the number of individuals would significantly decrease and the survival rate would strongly decline in the medium with propolis concentration at 2.40 mg/ml.