Objective: To observe the influence and study the mechanisms of Xiaoyaosan (XYS) to
hippocampal nervous structural
synaptosome
of rats under multi-stress methods. Methods: Rats were randomly allocated to tive groups (normal control group, model group 1, model group 2, XYS group 1, XYS group 2). Model was selected according to Carts. Nissl body of Hippo-campal nerve-cell was detected by Nissl staining,
Hippocampal synaptosome were detected by Sevier-Munger staining. The concentration of hippocampal synaptic Ca 2+ ion was detected by fluorimetry. Results: Contrasted with normal control group, Nissl bodies and hippocampal synaptosomes of model group 1 rats decreased, it became disorder that the arrangement of model groups rats hippocampal synaptosome. Those change of XYS groups was less than model groups. Concentration of Ca 2+ ion in hippocampal synaptosome of rats, normal control group is less than the other groups (P<0.01); XYS group 1 is less than both model groups (P<0.01); XYS group 2 is less than XYS group 1 (P<0.05); model group 2 is also less than model group 1 (P<0.05). Conclusion: These observations suggest that the multi-sress model could damage hippocampal synaptic structure by increasing the concentration of Ca 2+ ion in hippocampal synaptosome of rats and XYS can decrease this kind of damage by restricting the concentration of Ca 2+ ion in hippocampal synaptosome of rats increasing.