BACKGROUND:To tonify qi,benefit spleen and primary qi,strengthen tendons and b ones and promote circulations in meridians and collaterals are the classical pri nciples in Chinese medicine for anti kinetic fatigue. OBJECTIVE:To observe the efficacy of compound Chinese herbal medicine on anti kinetic fatigue. DESIGN:Randomized controlled experiment based on animals. SETTINGS:Animal Experimental Center of Wenzhou Normal University and Zhejiang College of Traditional Chinese Medicine. MATERIALS:The experiment was performed in Animal Experimental Center of Zhejia ng College of Traditional Chinese Medicine from February to June 2001,in which,8 0 ICR mice of either sex,body weighted(22±2) g and 40 SD male rats,body weighte d(194±7) g were employed. METHODS:①Eighty mice were randomized into two groups,of which,40 mice was gas tric infused with Chinese herbal jiang li fang(experimental group) and 40 mice was with physiological saline(the control).After bred for 15 days,they were rand omized into 3 groups for anti fatigue experiment(n=15,swimming time),anti hypo xia ability(n=15) and determination of hemoglobin(n=10).②Determination of testo sterone:Forty rats were randomized into 4 groups,named the control with physiolo gical saline infusion,herbal infusion group,exercise group,exercise herbal infu sion group.In herbal infusion group,the rats were infused once a day.In exercise group and exercise herbal infusion group,the rats exercised once a day,swimmin g till being exhausted at the tolerance of 3%body weight at 28 ℃of water tempe rature.After bred for 20 days,heart blood 2 mL was collected with chest opened a nd the serum was centrifuged and placed in refrigerator at 4 ℃. Minigamma 1 275 γcounter and VCS method were applied for determination. MAIN OUTCOME MEASURES:①Determination of swimming time of mice in every group. ②Time of anti hypoxia.
③Levels of hemoglobin and testosterone in rats. RESULTS:Forty rats and 80 mice were all entered the result analysis.①Observat ion of swimming time in mice:The time was remarkably prolonged in experimental g roup compared with the control<(125.2±13.7),(108.9 ±12.6) minutes,P< 0.01>.②A nti hypoxia ability:It was much improved in experimental group compared with th e control<(25.0±1.5),(23.1±1.8) minutes,P< 0.05>.③Level of hemoglobin:The lev el of hemoglobin in experimental group was higher than that in the control,but t he difference was not significant<(162.1±5.2),(159.3±5.3) g/L>.④Level of test osterone:That in exercise group of rats was remarkably lower than exercise herb al infusion group<(4.69±0.92),(6.03±0.77) nmol/L, P< 0.05>.The difference in t estosterone level was not significant between the control with infusion of physi ological saline and herbal infusion group<(6.59±0.91),(6.98±0.88) nmol/L,P >0. 05>. CONCLUSION:Under fatigue state,jian li fang can prolong the time of swimming i n mice,improve work time and efficacy,increase hemoglobin content and benefit an ti hypoxia ability,which indicates rather strong anti fatigue action.The combi nation of Chinese herbal jian li fang and exercise training can much significant ly increase serum testosterone level in rats compared with exercise group,which explains that jian li fang can increase testosterone level in rats,prevent testo sterone decrease induced by exhausted exercise and accelerate the recovery of ph ysical function.