Objective To study the acute skin toxicity, the skin irritation and analgesic effect of
guzhizengsheng complex plaster. Methods
① Sixty rats were randomized into six groups evenly:A 1, A 2, B 1, B 2, control one and control two (n= 10), each one with 40 cm 2 molted on both sides of backbone to take the acute skin toxicity test. A 2, B 2 and control two were used as the skin-impaired models. A 1 and A 2 were given
guzhizengsheng complex plaster, B 1 and B 2 were given the plaster basis. The systematic toxicity and topical toxicity were observed. ② Six rabbits were randomized into two groups, each one with 50 cm 2 molted on both sides of backbone to take the acute skin irritation test. The left areas were given 1 g
Guzhizengsheng complex plaster daily, sequentially for 7 d, and the right areas given the plaster basis in the same way. And the irritation was observed and graded. ③ Forty mice with a pain threshold of 6 s were randomized into four groups: Ⅰ, Ⅱ, Ⅲand Ⅳ. Group Ⅰand group Ⅱ were separately given 1. 5 g and 3. 0 g guzhizengsheng complex plaster daily, for 3 d. Group Ⅲ were given 3. 0 g guzhizengshengyitieling, and group Ⅳ given 3. 0 g plaster basis. On the forth day, their pain thresholds were tested at 30, 60, 90, 120 min. Results ① There were three rats died in A 2 group, and one in B 2 group. And there were no abnormality found in the live rats. ② The scores on normal skin and on impaired skin were 1.40, and 2.00 by guzhizengsheng complex plaster; and 0.30 and 0.30 by the plaster basis. The irritation is slight to normal skin and moderate to impaired skin by degree analysis, and the plaster basis had no irritation to normal skin and impaired skin.③ By comparison to group Ⅳ, the pain thresholds in group Ⅰ, Ⅱ, and Ⅲ were significantly increased (P<0. 01). Conclusion The guzhizengsheng complex plaster and the plaster basis have slight toxicity and irritation on the normal skin, and light toxicity and moderate irritation on impaired skin.Guzhizengsheng complex plaster can increase the pain threshold and has the value to develop.