Objective: To investigate the effects of four different components 〔curcuma aramatica oil(CAO),curcumol, βecement and curcumin〕
of Ezhu(莪术) on the gene expression of hepatic stellate cellsT6(HSCT6). Methods: The mRNA sequences of 50 liver fibrosisrelated genes were looked up from a gene bank and oligonucleotide probes were designed by software of designing oligonucleotide probe. Oligonucleotides were synthesized PE8909DNA synthesizing instrument. OGR04 dropping instrument and aldehyded glass chip were used for the preparation of oligonucleotide microarray. Cultured HSCT6 cells were treated with different concentrations of colchicines, CAO, curcumol, βecement and curcumin. According to the experiment of cell toxicity, the appropriate concentrations of medicine used were determined by the survivor rate more than 50%. The blank control of each group was set up and the cells were collected at 1, 6, 12 and 24 hours respectively. According to Trizol manua, Trizol was applied in the extraction of the total RNA. The labeled cDNAs were prepared from the total RNA by the reverse transcription fluorescein labeling method, and in the preparatory process Cy3dUTP and Cy5dUTP were applied. These labeled cDNAs hybridized with the oligonucleotide microarray was rinsed for a few times. Afterwards, The gene microarray was scanned by scanner Genepix 4000B and the different gene expressions of HSCT6 cells were analyzed with Ima Gene 4.2 software. Results: After HSCT6 cells were cultured in a medium with 6.25 μg/ml of colchicines for 12 hours, gene expression of tissue inhibitor of metalloprotenase 1(TIMP1) was downregulated by 220%, and in a medium with CAO 78.125 μg/ml for 24 hours, gene experessions of TIMP2 and interleukin6(IL6) were downregulated by 230% and 220%,respectively. After HSCT6 cells were cultured in a medium with curcumol 1.562 5 μg/ml for 12 hours, the gene expressions of transforming growth factorβ_1(TGFβ_1)and P450a were downregulated by 230% and 210%, respectively. Conclusion: These results show the possible mechanism of antihepatic fibrosis of CAO and βecement are on the molecular level.