Effect of edaravone on expression of oxidoreduction protein in brain tissue of rats after focal cere

AIM: To study the protective effect of edaravone(MCI186),a free radical scavenger,on brain injury by investigating the effects of MCI186 on expression of oxidoreduction protein in brain tissue and infarct areas in experimental rats after focal ischemia.METHODS:The experiment was performed in the Institute of Neurology, Zhujiang Hospital of Southern Medical University from May to September 2004.Totally 60 healthy male SpraqueDawley rats were divided randomly into MCI186 and control groups (n=6),the rest 48 rats were divided into normal, sham operation, 1, 6, 12, 24 and 48 hours of cerebral ischemia and MCI186 groups (n=6), and they were observed by using immunohistochemistry method. Rats expect in normal and control groups were established models by intraluminal filament occlusion of middle cerebral artery. Rats in two MCI186 groups were injected with MCI186 (0.3 mg/ kg) into caudal vein 30 minutes and 12 hours following cerebral ischemia.TTC staining and immunohistochemistry method were used to evaluate the effect of MCI186 on infarct area and expression of apurinic/apyrimidinic endonuclease (DNA prosthetic multifunctional protease)/oxidoreduction factor1 protein in ischemic penumbra in ischemic brain tissue. RESULTS:Finally 60 rats were involved in the result analysis.①The infarct area decreased significantly in MCI186 groups as compared with that in control group <(31.2±2.3), (46.5±8.6)mm2, t=4.236, P=0.002>.②The expression of apurinic/apyrimidinic endonuclease/ oxidoreduction factor1 protein in ischemic penumbra in MCI186 groups decreased at hour one following ischemia, and reached to the peak at hour 48, and there was significantly difference as compared with that in normal group<(25.9±4.7), (74.8±7.3)/mm2, F= 99.4, P=0.000 297>.MCI186 could increase its positive expression <(45.8±4.3) /mm2, P< 0.05>.CONCLUSION: Through upregulating the expression of apurinic/apyrimidinic endonuclease, MCI186 can improve the repair ability of brain tissue in the course of ischemic injury, decrease the infarct area, so as to protect ischemic brain injury.