AIM:To study effects of Qi salt on the formation or excretion of uric acid. METHODS :①Studies on xanthine oxidase(XO) inhibitory
activity of Qi salt in vitro ;②Effects of Qi salt on serum uric acid level and excretion in hyperuricemia rats model induced by
potassium oxonate; ③A
excretion test of phenolsulfonphthalein (PSP). By vitro and vivo experiments Qi salt has been evaluated for effects on the formation or excretion of uric acid. RESULTS: ①At the concentration of 100 mg·L -1 the inhibitory activities of Qi salt and sea salt on XO was 40.5% and 12.7% respectively. At the concentration of 50 mg·L -1 the inhibitory activities of Qi salt and sea salt on XO was 29.7% and 3.17% respectively. The IC 50 value of Qi salt was 174.7 mg·L -1 and the IC 50 value of allopurinol used as a positive control was 1.07 mg·L -1 . The dose effect relation was observed.② By treatment with Qi salt (1.6, 0.8, 0.4 g·kg -1 ) the serum uric acid level of hyperuricemia rats model pretreated with potassium oxonate was significantly decreased during 1 5 h ( P <0.01 or P <0.05). The dose effect relation was observed. From the data, the total amount of excretion of the urine and uric acid was significantly increased( P <0.01). ③In the test of PSP excretion no effect was observed for reabsortion and secretion of the renal proximal tubule by Qi salt(0 1-0 4 g·kg -1 ). It suggested that Qi salt possessed not uricosuric activity. CONCLUSION: The inhibitory activity of Qi salt on XO was observed in vitro . The serum uric acid level of hyperuricemia rats model was decreased significantly and the dose effect relation was observed by treatment with Qi salt. Uricosuric activity was not observed by treatment with Qi salt in excretion test of PSP.