Objective: To explore the effect of L158, 809 (angiotensin Ⅱ receptor blockers, ARBs) and Cilazapril (Angiotensin converting enzyme inhibitor, ACEI) on the ex-pression of transforming growth factor-β1 ( TGF-β1 ) and secretion of fibronectin, laminin and type Ⅳ collagen from the cultured human mesangial cells . Methods: Human mesangial cells were cultured in different glucose (5.6 mmol/L and 30 mmol/L) and agents (1, 10, 100 and 500 μmol/L) concentrations . The proliferation of mesangial cells were detected at 24, 48 and 72 h. Then the mesangial cells are divided into four groups, low glucose (5.6 mmol/L) control group, high glucose (30 mmol/L) control group, L158,809 (10 μmol/L) group and cilazapril (10μmol/L) group. Forty-eight hours later, the expression of TGF-β1 were detected by RT-PCR. Concentrations of TGF-β1, fibronection, laminin and type Ⅳ collagen in the su-pematants of the mesangial cells were determined by ELJSA and radioimmunoassay methods. Results : Compared with low glucose control group, the mesangial celis under high glucose me-dium show excessive proliferation and more TGF-β1, fibronectin, laminin and type Ⅳ collagen in the supernatant. The expressicn of TGF-β1 mRNA was also significantly increased under high glucose. The levels of TGF-β1 and ECM ( extracellular matrix ) proteins in the L158, 809 group and cilazapril group are obviously lower than that of the high glucose control group . The expression of TGF-β1 mRNA was markedly decreased in the L158, 809 group and dlazapril group com-pared with that of high glucose control group . Con-clusion : High glucose stimulated the cultured human
mesangial cells to excessively proliferate, express TGF-β1 and secrete ECM proteins , and the high glucose-induced changes were suppressed by either L158,809 and cilazapril.