OBJECTIVE The effect of Zedoary Turmeric Oil Gelatin Microspheres(ZT GMS) on rat's nervous system as well as canine respiration and cardiovascular systems were observed, and it's safety for intervention therapy was probed. METHOD To consider the effect of ZT GMS to rats' psyche nerve system by stomach duodenum artery cannula to proper hepatic artery modelling interventional therapy. Rats' general behavior and ability to walk a 90 cm long×2.5 cm wide wood beam were observed after ZT GMS administered through proper hepatic artery. The effect of ZT GMS to respiratory system and cardiovascular system were recorded by six channel physiological recorder after ZT GMS administered from femoral artery to proper hepatic artery under x rays and arterial contrast.RESULTS ZT GMS doesn't affect rat's general behavior, posture, gait and pupil. There is no salivation and muscular tremor in rats. In the test of ability to walk the wood beam, the falling of times is less than three times every 3 minutes. Also, ZT GMS doesn't affect respiratory frequency, blood pressure and heart rates of dogs.CONCLUSION The study indicated that intervention therapy of ZT GMS from hepatic artery has no obvious effects on respiratory, cardiovascular, and nervous systems. It exerts only local action, while the systemic side effect is little.