AIM To investigate the effects of
tetrahydroxystilbene on binding force of NMDA receptors and concentration of intracellular
calcium ions of nerve cell in brain ischemia reperfusion rodent and to explore the mechanism of protection of
tetrahydroxystilbene on brain. METHODS Gerbil and mice's bilateral carotid arteries were occluded for 10 min after anesthesia and then reperfused to make ischemia reperfusion model:(1) Gerbils were sacrificed 7 d after reperfusion and the binding force of NMDA receptors of gerbil forebrain were assayed; (2)Mice were sacrificed 15 min after reperfusion and their brains were sliced. Fluo 3/AM was added into the slice and fluorescent intensity of intracellular free calcium in cortex and hippocampus area of different layer were observed through optical section of
laser scanning confocal microscopy (LSCM). RESULTS Binding force of NMDA receptors of model group was significantly higher than that of sham operation group, while tetrahydroxystilbene treatment can decreased it significantly. Optical section result showed that calcium concentration in model group was higer than that of sham operation group. Compared with model group, the calcium concentration in the group treated with tetrahydroxystilbene was lower remarkably. CONCLUSION Tetrahydroxystilbene may relieve the increase of binding force of NMDA receptor and the raise of intracellular calcium ion concentration of rodent nerve cell caused by brain ischemia reperfusion, thus alleviate brain injure induced by calcium overload, and have protective effects on brain.