AIM To study the protection of str agalus membranaceus on
intestinal mucosa and its mechanism during
hemorrhage shock in
rabbits. METHODS 32 rabbits were randomly divided into four groups:Group Hh(n =8), used Astragalus membranaceus (10 g·kg -1 ); group Hs(n=8),used A stragalus membranaceus (5 g·kg -1 ); group S:n=8, used Anisodamine (2 5 mg·kg -1 ); group C(n=8), used saline as therapeutic drugs. The small intestinal moucosa
microcirculation blood flow, MAP, CVP and HR were measured d uring
hemorrhage shock. The structure of small intestine tissue in 10 cm to ileu m distal end was observed by Electron microscope after experiments. SOD activity and MDA concentration of intestinal mucosa were detected after experiment. RESULTS ①During hemorrhage shock, MAP and CVP decreased compared wi th pre-shock levels in each group(P<0 01).②During hemorrhage shock, sm all intestinal mucosa microcirculation flow decreasd obviously in each group; At 20, 30, 40,50,60 minute after blood withdrawing, the values of small intestina l mucosa microcirculation blood flow in group Hh and group S were higher than t hat in group C(P<0 05 or P<0 01), there were no significant differe nce between group Hs and group C(P>0 05). At 40,50,60 minute after blood withdrawing, the values of small intestinal mucosa microcirculation blood flow in group Hh were higher than that in group Hs. ③Chiu's score of small intestina l structure in group Hh and S were very significantly lower than that in group C (P<0 01), and the score in group Hs was significantly lower than that in group C(P<0 05).The Electron microscope pathlogical results and three-di mensional structural parameters of the mitochondrion showed:intestinal mucosa ep ithelial cells damage was severe in group C, medium in group Hs, slight in gr oup Hh and group S. ④Compared with group C and group Hs, the SOD activity of in testinal mucosa increased and the MDA concentration of intestinal mucosa decrea sed in group Hh and group S (P<0 05). CONCLUSIONS Stragalus membranaceus can reduce the intestinal mucosa epithelial cells damage by increa sing intestinal mucosa microcirculation blood flow and reducing oxidation injury . Its effect is similar to that of Anisodamine.