To develop a high performance liquid chromatography ( HPLC) - mass spectrometry ( MS)
method for the determination of flunarizine in human plasma. Methods: The
plasma was alkalized with 1 mol . L-1 sodium hydroxide solution and extracted with ether - hexane (2: 1). The separation was performed on a Diamonsil C18 analytical column using methanol - water(70:30,containing 1% formic acid)at a flow rate of 0. 4 mL . min-1 as the mobile phase. The analytes were detected by the positive electrospray ionization( ESI+ ) - MS method under selected -ion monitoring (SIM) mode. The MS
detection parameters was set as follows: the
drying gas flow was 13 L . min -1 , the drying gas temperature was 3 5 0 C , the nebulizer pressure was 0.21 MPa , the capillary voltage was 4 000 V, the fragmentor was 150 V and the SIM ions were m/z 405. 2 < M + H > + for flunarizine and 369. 2 < M + H> + for cinnarizine. Results:The linear range of the plasma concentration of flunarizine was 1 ~ 100
ng . mL-1, and the limit of detection was 0. 2 ng . mL-1. The relative recoveries of flunarizine were 96. 5% -101.8%. The RSDs of intra - day and inter - day were less than 5. 0% and 14. 0% , respectively. Conclusions: The method has high sensitivity and good selectivity, it can be used for the pharmacokinetic research of flunarizine.
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