Aim To develop a sensitive and specific
liquid chromatography tandem mass spectrometry (LC/MS/MS) method for the determination of
adefovir in monkey plasma.
Methods Adefovir and internal standard 9 (3 phosphonylmethoxypropyl)adenine were isolated from plasma by protein precipitation with methanol, then chromatographed by using a Diamonsil C 18 column. The mobile phase consisted of
methanol water formic acid (20∶80∶1). Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Selected reaction monitoring (SRM)
mode with the transitions of m/z 274→ m/z 162 and m/z 288→ m/z 176 were used to quantify adefovir and the internal standard, respectively. Results The linear calibration curve was obtained in the concentration range of 0 02- 4 00 mg·L -1 . The lower limit of quantitation was 20 μg·L -1 . The inter and intra day precision (RSD) was less than 5 8%, and the accuracy (relative error) was within ±4 5%. The method was successfully used in a pharmacokinetic study of
adefovir dipivoxil in monkeys. Conclusion The method is proved to be suitable for pre clinical investigation of adefovir
Dipivoxil pharmacokinetics, which offers advantages of specificity and simple sample preparation compared with the previously reported methods.
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