Object To evaluate the effects of
ginsenosides (GSS) in stem and leaves of ginseng on
glucocorticoid receptor (GR) in the
hemorrhagic shock rats, and study the mechanism. Methods Rats were divided into hemorrhagic shock group and control group. The rats in hemorrhagic shock groups were ig 200, 100, 50 mg/kg/d GSS, model group and control group were ig distilled water 2 mL for 10 days. The Rs of
GR in brain and
hepatic cytosol of rats were measured by radioligand binding assay, using < 3H> dexamethasone as the ligand. The level of GR mRNA expression in hepatic cytosol were determined by RT-PCR. Plasma adrenocorticotrophic hormone (ACTH) and glucocorticoid (GC) concentrations were determined by the radioimmunoassay. Results Rs of GR in brain and hepatic cytosol were higher in hemorrhagic shock+GSS groups than those in hemorrhagic shock group, and the Rs of GR was the highest in hemorrhagic shock+10 mg/mL GSS group (P<0.01); Expression of GR mRNA in hepatic cytosol was higher in hemorrhagic shock+GSS groups than those in hemorrhagic shock group; The plasma concentrations of ACTH and GC were not altered by GSS. Conclusion GSS can alleviate the decrease of the Rs of GR in brain and hepatic cytosol through the way to accelerate the expression of GR mRNA. Perhaps the 10 mg/mL is the best dosage of ginsenosides.
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