AIM: Studies on the
Antiendotoxic mechanism of
Radix Isatidis . METHOD: The content designed has finished on the basis of the facts identified by lots of experiments that the chloroform part (F02)from Radix
Isatidis and F022(isolated from F02) to facture solution 1%(sample) . The
endotoxin was shown to have change with the effect of solution on the electron mierocope ;Using coloration matrix as a method for the quantitation of endotoxin , pretreated by sample ; the delease of the inflammation factors stimulated by LPS was inhibited the influences on the molecular expression of moesin mRNA in mice tissues and MAPKp38,TNFα,IL6 and NO in mice for LPS 's effect of inhibition , studies on the Antiendotoxic mechanism of Radix isatidis . RESULT: With the sample of Radix isatidis effect to have a long chain and then to ture pieces with effect of solution ; It was found that 20EU of the endotoxins could be reduced from 20EU to 0.02EU by 0.5ml of 1% F02 solution and the reduction rate was 99% ; 1% F02 and 1% F022 (20 ng/ml) to 50 and 100 ng/ml concentration for the inflammation factors stimulated by LPS with excreled TNFα,IL6's
restrain rate was 84.4%,49.0%,77.6%,42.4% and 78.6%,41.7%,85.9%,39.6% ;1% F022 stimulated mice's liver,kidney,spleen molecular expression of moesin mRNA stimulated by LPS was 93.5%,88.6% and 87.8%,restrain rate was 90.1% for average;1% F022 stimulated MAPKp38,TNFα,IL-6 and NO in mice stimulated by LPS was 93.2%,96.6%,95.8% and 93.0%. CONCLUSION: The chloroform part (F02) from Radix Isatidis and F022 not only the endotoxin was changed but also give voice to restrain the delease release with transition both in bodyside , restrain extend for film albumen and enzyme , clarify the mechanism on the Antiendotoxic Action of Radix Isatidis from level with molecule .
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