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Shvoong Home>Medicine & Health>Studies on determination of cefpodoxime in plasma by HPLC and its pharm acokinetics in human Review

Studies on determination of cefpodoxime in plasma by HPLC and its pharm acokinetics in human

Article Review   by:TsingHua     Original Author: Chinese Pharmaceutical Journal
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OBJECTIVE A HPLC UV method was established for th e determination of cefpodoxime in plasma and was applied to the pharmacokinetic study of cefpodoxime in 20 volunteers.METHODS The chromato graphic separation was achieved on a Kromasil C 18 (150 mm×4.6 mm,5 μm), the mobile phase was consisted of water acetonitrile triethylamine(1000∶136∶ 5)(adjusted to pH=4.6 with phosphoric acid),The detection wavelength was at 2 54 nm.The internal standard was cefaclor,The plasma samples were extracted with solid phase extraction and the urine sample were dilute 10 times.RES ULTS A good linearity was obtained from 0.050 to 6.400 mg·L -1 in plasma with a correlation coefficient of 0.999 7 and from 0.2 to 25.6 mg·L -1 in urine with a correlation coefficient of 0.999 9,respectively.The recover y of plasma extraction was more than 79%.The within day and between day RSD we re less than 12.0% .The pharamacokinetic analysis of cefpodoxime after oral a dministration of 200 mg cefpodoxime proxetil tablets was studied.It was found to be fitted to a one compartment open model and its pharmacokinetic parameters w ere as follow: t max (3.0±0.4) h, c max (3.4±0.4) mg·L -1 ,AUC 0~t (17.7±2.1) mg·h·L -1 and V (37±6) L,respectively. The accumulation elimination rates of cefpoxime in urine were(47.8±2.9)% after 12 h.CONCLUSION The pharmacokinetics of cefpoxime in human p rovided a useful index for clinical trial.
Published: February 15, 2003   
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  1. Answer   Question  :    retention time of cefpodoxime and cefaclor in human plasma hplc analysis View All
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