OBJECTIVE To investigate the acute effect of
cyclosporine A,gentamycin and
selenium preparation on the expressions of molecules
and genes from peripheral lymphocytes in rats.METHODS 24 normal wistar rats were divided into control and 3
experimental groups.The experimental groups were orally administered with cyclosporine A (CsA),gentamycin and
selenium preparation for two days respectively.The peripheral Lymphocytes were isolated and reacted with mono-clone antibodies for labeling molecules of MHC-II,ICAM-I,CD4,CD8 and IL-2R total mRNA in Lymphocyte were extracted.The expressions of molecules and genes were separately evaluated by flow-cytometer and differential display technology.The CD4 + and CD8 + lymphocytes in the myocardium were counted by immuno-histochemistry.RESULTS Compared with the control group,the expressions of CD4,CD8 and ICAM-1 in three experimental groups were all down-regulated,and accompanied with decreased myocardial infiltrations of CD4 + and CD8 + lymphocytes.CD4 + lymphocyte infiltration was significantly correlated with CD4 molecules expression (P<0.0001).The reductions of the molecules and lymphocyte infiltrations in experimental groups trended to CsA>gentamycin>Selenium.In contrast down-regulating expressions of MHC-Ⅱ and IL-2R in CsA group,gentamycin group exhibited to up-regulate MHC-Ⅱ and IL-2R,and selenium preparation group displayed to down-regulate MHC-Ⅱ and up-regulate IL-2R.An apoptosis gene-gig18 mRNA was up-regulated.CONCLUSION ①Short-term administration of CsA,gentamycin and selenium preparation inhibited peripheral lymphocyte expressing CD4,CD8 and ICAM-1 by gradualness.②Three experimental groups appeared different regulating mechanism in MHC-Ⅱ and IL-2R expressions.③An apoptosis gene-gig18 mRNA was up-regulated in all experimental groups.