ABSTRACT:OBJECTIVE To explore the effects of Bushentongmai recipe on
insulin receptor expression in skeletal muscles
in type Ⅱ diabetic rats.METHODS The male healthy Wistar rats were given a low dose streptozotocin intravenously and fed with high calorie laboratory caw to establish the murine model of type II diabetes. The animals were randomly divided into model group,Chinese medicine group and metformin group (giving Bushentongmai recipe and metformin for 8 weeks respectively).The rats fed with normal laboratory caw were designated as normal group. The amount of skeletal muscle triglycerides (mTG) was determined enzymatically. The
insulin receptor (InsR) mRNA expression level was evaluated by reverse transcription polymerase chain reaction (RT PCR).RESULTS Compared with normal group,the mTG content in model group was significantly elevated ( P < 0.01 ),while the express of muscular InsR mRNA was remarkably decreased ( P < 0.01 ).In comparison with model group, the murine mTG in Chinese medicine group was obviously reduced ( P < 0.01 ),but the express of muscular InsR mRNA was significantly increased ( P < 0.01 ).CONCLUSIONS It is suggested that the mechanism of Bushentongmai recipe on improving
insulin resistance in type 2 diabetic rats might be closely correlated with its up regulating the muscular InsR mRNA expression.