Objective To investigate effects of
Euphorbia fisheriana stead to cure T cell leukemia caused by retrovirus. Methods
To divide 50 mice of 615 strain into 5 groups, leukemia group (Ⅰ), two treated groups (Ⅱ & Ⅲ),
Euphorbia fisheriana stead control group without leukemia (Ⅳ), and normal group (Ⅴ). 615 mice from Ⅰto Ⅲ groups were inoculated by leukemia cells with
retrovirus. Then Ⅱ, Ⅲ and Ⅳ group were given Euphorbia fisheriana stead water lixivium, the dosage was 1.8?g/kg, 3.0?g/kg, and 3.0?g/kg, respectively, orally once time a day for 1 week. The total number of WBC in peripheral blood was counted and apoptosis characteristics of tumor cells, such as apoptosis body and DNA electrophoresis were observed. c-myc and ras gene expressions were detected by histrochemical method. Results WBC number of treated group with 3.0?g/kg <(0.116?4± 0.012?3 )×109/L> was much lower than tumor group <(0.219?9±0.109?9)×109/L, ( P <0.01)>. The apoptosis rates in tumor cells of two treated groups <(23.60±2.27)%,(36.40±4.99)%> were higher than tumor group <(4.60±0.97)%> and P<0.01. The typical apoptosis morphology and DNA trapeziform stead were appeared in tumor cells of two treated groups. To compare with tumor group, the expressions of c-myc and ras in Euphorbia groups were less ( P <0.01).As the dosage increased, the effect of therapy was more remarkable(P< 0.01 ). Conclusion Euphorbia fisheriana stead can inhibit T leukemia cell proliferation and so is potential by promoting apoptosis and inhibiting c-myc/ras expression. <