Objective To investigate pharmacokinetic parameters of
panipenem in patients with respiatory disease, an HPLC
method was proposed for assaying panipenem concentration in human
plasma and in ureine . Method 1mol/L 3 (N mopholine) propanesulfonic acid (pH7 0) was used as stabilize of panipenem, paracetamol was used as the internal standard of plasma. The samples were chromatographed on a reversed
phase C 18 column (5μm, 250mm×4 6mm) using a mobile phase of 0 04mol/L ammonium acetate (pH4 0)-methanol (90∶10, V/V ) at a flow
rate of 1 6ml/min for plasma, while the mobile phase for urine was 0 04mol/L ammonium acetate (pH4 0)-methanol (98∶2, V/V ) at a flow rate of 1 6ml/min. And the drug was detected by UV chromatography at wavelength 299 3nm. Results The linear range of panipenem concentration in plasma was 0 5～50 0μg/ml ( r =0 9999) and the detection limits was 0 2μg/ml, the avarage recovery rate of panipenem concentration 2 0, 10 0, 50 0μg/ml was 98 69% ( n =6), the RSDs inter day and between days were 1 92%, 2 44%, 2 31% ( n =5) and 2 15%, 1 45%,2 68% ( n =3), respectively. The linear range of drug concentration in urine was 2 0～200 0 μg/ml ( r =0 9999) and the determination limits was 1 0μg/ml, the avarage recovery rate of panipenem concentration 10 0, 25 0, 100 0μg/ml was 101 30% ( n =6), the RSDs of inter day and between day were 2 63%, 1 05%, 0 88% ( n =5) and 2 30%, 3 85%, 2 38% ( n =3), respectively. Conclusion This method was simple, rapid, accurate, and suitable for routine clinical determination of paniaenem.
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