Objective\ The investigate the effect of polypeptide isolated from Chlamys Farreri (PCF) on Hela
cells damaged by ultraviolet(UV).\ Methods\ The suspension of Hela epithelia cells (5×10 8/mL ) cultured for 24 hours were prepared and 1 mL of it was put into each hole of 24 hole plates. Some Hela cells were randomly divided into six groups: control
group (C), modal group(M), 5 g/L PCF group, 10 g/L PCF group, 20 g/L PCF group and 10 g/L Vitamin C (Vc) group. After incubation with RPMI 1640, PCF, Vitamin C, respectively, for 10 minutes, the cells were exposed to UVA ( irradiation intensity: 3 650 μJ/cm 2), with the exception of the mice in C group. Some other Hela cells were divided into another six groups, they were treated almost the same as above, but irradiated by UVB ( irradiation intensity: 7.15×10 -5 J/cm 2). The
intracellular free calcium (Ca 2+ ),
apoptosis and
Cell death rate were measured by flow cytometry (FCM). Enzyme
activities and malondialdehyde (MDA ) were determined by biochemical assays.\ Results\ The apoptosis and cell death rate were decreased and the contents of intracellular
ca 2+ and activities of succinate dehydrogenase (SDH) as well as the activities of GSH px, SOD,CAT increased and the MDA decreased by the action of PCF. \ Conclusion\ It is suggested that PCF could protect the cells from the damage caused by UVA and UVB by enhancing the activities of antioxidant enzymes.
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