Objective:To study the expression of Bclˉ2and Bax in rat hepatic fibrosis model and the role of
HuoˉXueˉShenˉShiˉFang(HXSSF).Methods:Hepatic
fibrosis was successfully induced in rats by subcutaneous injection of
carbon tetrachloride(CCl 4 ),the rats were divided into Fibrosis model group,FuˉFangˉBieˉJiaˉRuanˉGanˉTablet postive control group and HXSSF treatment group(10gkg -1 d -1 ,i.g.for12weeks),normal control group is another group of10rats without any treatment.At last,Bclˉ2and Bax proteins were detected by immunohistochemistry.Results:Bclˉ2was exˉpressed weakly in the plasma of hepatocytes and hepatic sinusoid in normal control,but it was expressed strongly in portal area,fibrous septae,hepatic sinusoid,the plasma and membrane of hepatocytes and central vein in rat fibosis model(P<0.01).Bax was expressed slightly in central vein and the hepatic sinusoid around,it was also expressed strongly in the plasma of hepatocytes,hepatic sinusoid,fibrous septae,membrane of hepatocytes and epithelial cells of bile duct(P<0.01).Compared with that in fibrosis model induced by CCl 4 ,the expression of Bclˉ2was sigˉnificantly lower in rats treated with HXSSF(P<0.01),but it was not significantly different in fibrous septae(P<0.01),the expression of Bclˉ2was significantly lower in rats treated with HXSSF(P<0.05),and it was significantly lower in fibrous septae(P<0.05)too.Conclusion:The expression of Bclˉ2and Bax was increased in rat hepatic fibrosis model.HXSSF can promote the apoptosis of liver cells in fibrous septae,and promoting myofibroblast cells apoptosis is possibly one of the mechanisms of treating hepatic fibrosis.