The effects of
Jiunaoning Injection (JI) on neuron apoptosis induced by hypoxia and
hypoglycemia were studied. SH SY5Y was
used to establish the model of neuron apoptosis induced by hypoxia and
hypoglycemia. Flow cytometry and living cell fluorescent double staining were used to observe the neuron apoptosis and the inhibitory effects of JI on the neuron apoptosis. The results showed that neuron apoptosis could be induced by the hypoxic and hypoglycemic culture, and an obvious apoptotic peak in front of the G1 peak with an apoptotic ratio of 42 99%±7 73% was found by the flow cytometry analysis; after the fluorescent double staining was performed, the coacervation of cytoplasm in the apoptotic neurons was observed by the fluorescence microscope. The results also showed that a medium or a large dose of JI could decrease the apoptotic ratio of the neuron in the JI treating group; and there was a significant difference between the result from the JI treating group and that from the model group ( P <0 01), and an insignificant difference between the result from the JI treating group and that from the nerve growth factor group ( P >0 05). Therefore, it could be concluded that JI can inhibit the neuron apoptosis induced by hypoxia and hypoglycemia, and possesses protective effects on the neuron.