DISCOVERING OF WHITE (PROTEIN) will will emerge in URINE OF ATTEMPT (TEST) by boiling QUALITATIVE 1.Próba and acidulation.
Principle of method this attempt (test) is simplest attempt of (test of)
discovering of white (protein). White (protein) undergoes influenced by temperature dęnaturacji i.e. small parts are transformed to whites (proteins) hydrofilowe hydrofobowe, which (who) in point koagulują izoelektrycznym. In order to it has come have for it reaction acid urine teddy-bear slightly koagulacji. This reaction is achieved by for urine of several bead of diluted acid added addition octowego. Acid protects before in alkaline urine of phosphate equally octowy cześnie wytrąceniem or węglanów. 2. Attempt (test) 20% acid ( )
Principle method Mac Wiliama sulfosalicylowy in solutions below point group white (protein) izoelektrycznego dysocjują aminowe and salts give with (from) anionami, especially they are at that connection with some for in research from the point of view of big affection of reaction it organic acids white (protein) take advantage solution ( undergo ) find (be placed) employment anionami nierozpuszczaIne.Kwas sulfosalicylowy odbiałczania denaturacji .Kwas sulfosalicylowy. White (protein) allows to discover about concentrating 0.015 15 ( ) 3 g/dl mg/dl. Attempt (test) Hellera. Principle of method nitrogen acid is concentrated white (protein) one of reagent strącających. Given a slight coloring to ring emerges on border of phase of connecting (adjoining) with acid urine yellow. This attempt (test) is less fond (sensitive) than attempt (test) Mac Williama, it takes a stand at concentrating white (protein) 0,033% only ( 33 ) mg/dl. On discovering white (protein) 4.Próba ortostatycznego. Principle of method on chill acid Albumozy wytrącają octowym. Method principle method EXTONA white (protein) with (from) solution of acid soda wytrąca sulfosalicylowym siarczanem, emerged intensity is defined at length of wave measurement gauged zmętnienia absorbancji 445nm. DISCOVERING OF GLUCOSE in URINE OF ATTEMPT (TEST) QUALITATIVE 1.Próba Fehlinga. Principle of method after confounding F and 7% ( ) CuSO4 and complex relationship (association) is built (is created) in (to) II ( ) F winian sodowo-potasowy NaOH, which (who) leads which (who) fall out (should) in connection with glucose reduction creating for oxide in the form of orange ( ) 2 Cu OH miedziawego Cu2O or depending on amount of glucose redly -orange precipitate. Principle method 2.Próba Nylandera it proceeds in reagent influenced by reductive relationships (associations) in (to) in the form of black -russet precipitate included (reach; make) metal Azotan bizmutu Nylandera bizmut. 3.Próba Benedicta. Principle of method glucose reduces copper siarczan, this way, degree of reduction is made public different paints quantitative designation of glucose enabling semi tail . Reagent is not by Benedicta reduktorem kreatyninę neither by acid moczowy. Qualitative method. Principle of method glucose consuming small part Heksokinazy fosforyzują ATP. It is oxidized simultaneous by with (from) OVER reduction emerging + glukozo-6-fosforan dehydrogenaze. It is proportional after revolt at length of wave for concentrating glucose in urine fellow incrementation (growth) which (who) NADH absorbancji 340nm. DISCOVERING in URINE attempt (test) INDYKANTÓW Obermayera and principle method Jollesa discovering is based on this reaction of split of (wreck of) relationship (association) on indykanu indoksyl and proper acid. Emerged is oxidized behind assistance ( indoksyl FeCl3 H2O2, ) KmnO4. Dye has been fabricated in next phase behind assistance indygo wyskstrachowany chloroformu, which (who) colors be on blue in correct urine most often ( him (it) ) 20mg/24h. DISCOVERING OF RELATIONSHIP (ASSOCIATION) in URINE attempt (test) KETONOWYCH Legala and attempt (test) Langego. DISCOVERING in URINE principle KRWII metoy attempt (test) will will emerge with (from) on other relationships (associations) which (who) characteristic tinge -magenta ( ) relatively own ability transmit (reschedule) oxygen undergo oxidize cause ( blue ) piramidynowa Hemoglobina hemaytna nadtlenków. Oxygen has been transmitted (rescheduled) on on hydrogen nadtlenek niramidon and it oxidizes him (it) for colorful relationship (association). DISCOVERING in URINE principle of method UROBILINOGENU characteristic red tinge reacts with (from) building (create) Urobilinogen odczynnikiem EHRLICHA. In case of (accidentally of) red revolt of tinge on chill, are said about amount zmożonej and we mean on ( + ), ( + + ), ( + + + ), ( + + + + ), if tinge it after warming correct amount emerge mean urobilinogenu and we record (write down) in norm „ ”. DISCOVERING in URINE attempt (test) principle method UROBILINY Schlesingera it relies on revolt of green fluorescence of zink salt urobiliny and sterkobiliny. As by prevent delete added addition blilirubina CaCl2 and ammonia zalkalizowanie. Precipitate of (settlement of) phosphate of limestone (calcium) Wytrąca, which (who) absorb bilirubin, we leak away urine. DISCOVERING OF BILIRUBIN in URINE 1.Próba Rosina. Principle of method it relies on oxidizing bilirubin behind assistance on green -iodine biliwerdynę. Fouchett 2.Próba. Principle of method it relies on oxidizing bilirubin on green behind assistance of reagent fouchett biliwerdynę. 3.Próba Gmelina. Principle of method bilirubin is oxidized in connecting (adjoining) with (from) strongly for several derivative oxidizing acid nitrogen green biliwerdyny, blue bilipurpuryny and yellow choleteliny. Rock-bottom 4.Próba Van Bergha. Principle of method bilirubin builds (create) complex relationship (association) with (from) in environment acid indifferent acid coloring slightly dwuazowanym sulfanilowym azobilirubinę or on black alkaline. DISCOVERING OF ACID in URINE attempt (test) .......it''s not end.