AIM To develop a
method for the determination of
trimebutine maleate in
rat plasma by using high performance
capillary electrophoresis. The method was employed to
pharmacokinetic analysis of trimebutine maleate. METHODS Plasma samples were deproteinized with acetonitrile (containing ephedrine hydrochloride as internal standard) and the supernatant was dried under N 2 stream at 50℃. The residue was dissolved with methanol water ( 1∶1 ) and injected into the capillary by siphon. The electrophoresis was performed in uncoated fused silica capillary and the voltage was 10 kV. The running buffer was 0 03 mol·L -1 NaH 2PO 4 (pH 6 0). The eluate was detected at 214 nm by UV detection. RESULTS The recovery for trimebutine
Maleate in rat plasma was 72 8%-87 9%. The calibration curve in plasma was linear over the range 5-200 μg·L -1 . The limit of quantitation was 5 μg·L -1 . The intraday relative standard deviation ( n =6) and the interday relative standard deviation ( n =18) were less than 14%. The highest concentration in plasma was observed at 30 min after ig trimebutine maleate to rats. The pharmacokinetic results were AUC 0-∞ =8 μg·min·mL -1 , T 1/2( K e) =173 min and K e=5 6×10 -3 min -1 . CONCLUSION The method is accurate, sensitive and suitable for pharmacokinetic study of trimebutine maleate.
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