OBJECTIVE A reversed phase high performance liquid chromatographic method was established for the determination of buflomedil in human plasma and was applied to the pharmacokinetic study of buflomedil in 12 healthy volunteers.METHOD The chromatographic separation was achieved on a Alltech C 18 column (200 mm×4.6 mm,5 μm),with methanol H 2O triethylamine(60∶40∶0.4 pH 6.5)as the mobile phase.The detection wavelength was at 275 nm.The internal standard was Diazepam. After alkaline with 2 mol·L -1 NaOH,the plasma was extracted with n hexane dichloromethane(3∶1).RESULTS A good linearity was obtained from 0.02 mg·L -1 to 4.00 mg·L -1 of buflomedil in human plasma with a correlation coefficient of 0.9995.The detection limit was 0.01 mg·L -1 .The extraction recovery was more than 93%. The within day and between day RSD were less than 12.0%.The pharmacokinetic analysis of buflomedil hydrochloride in 12 healthy volunteers after oral administration of 300 mg was studied.It was found to be fitted to a one compartment open model and its pharmacokinetic parameters were as follow: t max (1.58±0.29)h, c max (2.39±0.48) mg·L -1 , AUC 0～24 (17.68±4.66) mg·h·L -1 ,respectively.CONCLUSION The pharmacokinetics of buflomedil in human provided a useful index for clinical trial.