Observe and study the morphological changes and effects on myoleperoxidase(
MPO) of traditional Chinese medical complex Intestine clearing Suppository(ICS) in preventing and treating ulcerative colitis(
UC).Method: Divide the experimental
rats randomly into 6
groups,i.e.,blank,salicylazosulfapyridine(
SASP),model,high,middle and low
dosage of ICS,except the blank group,make other rats experimental UC model with albumen immunologic method of rabbit colon
mucosa,further divide them randomly into 2 experimental groups,i.e.,prevention and treatment,afterwards at 2nd~2lst day,give prevention group the medicine,22nd~35th day,treatment group,then decapitate them and take the colon tissue,and observe it morphologically under optical microscope,measure active MPO,analyse statastic results.Result: Through general morphological observation,we found there were serious congestion,edema,mucosa erosion,many large ulcers in the rats of model group;less serious mucosa erosion and quantity of ulcers in SASP and low dosage ICS groups:small ulcers,not serious mucosa erosion,low degree of congestion and edema in high and middle dosage ICS groups;the scores of inflammatory injury of ICS groups were markedly lower than that of model( P <0 01),meanwhile,the preventive and treating effectiveness of high and middle dosage groups was better than that of SASP( P <0 05).Under the optical microscope,there were large area of mucosa coloboma in the rats of model group,the ulcer penetrated to muscular layer and serous coat;in SASP group and low dosage ICS group,there were erosive mucosa and inflammatory cell infiltration,granulation on the base;in middle and high dosage groups,large part of colon mucosa recovered,granulation formed on the base,ulcers were obviously less than that in model group( P <0 01);there had marked difference compared with that in SASP group ( P <0 05).The MPO active values of high and middle dosage groups were markedly lower than that of model group( P <0 01).Conclusion: Traditional Chinese complex ICS had good prevention and treatment to rats UC,its effectiveness was positively correlated with dosage and better than that of SASP suppository.