To observe the histological and immunohistochemical changes in testis and epididymis after male mice in
immunological infertility
were treated with Chinese drugs mixture Mianbu Ⅰ & Ⅱ. By injection the antigen of mice sperm, model mice of
immunological infertility were made. The mice were fed with Mianbu Ⅰ, Mianbu Ⅱ, prednisone acetates or physiological saline in the same period of model establishment. The changes of histology and
immunohistochemistry of mice of immunological infertility were observed. Result: The level of antisperm antibody (AsAb) was high in serum and seminal vesicle plasma of male mice of immunological infertility. Immune complex mostly deposited in interstitial of testis? in limiting membrane and spermatogonium of convoluted seminiferous tubule of testis and epithelia of ductus epididymidis of epididymis. The number of spermatozoa and mature spermatid per convoluted seminiferous tubule decreased. However, the traditional Chinese medicine Mianbu Ⅰ & Ⅱ could significantly reduce the level of antisperm antibody and clear out the immune complex and regain the number of sperm and mature spermatid from seminiferous tubules. Conclusion: Traditional Chinese medicine Mianbu Ⅰ & Ⅱ can clear out circulating and partial AsAb, as well as immunological complex. Hence, they can increase the number of spem and mature spermatid from seminiferous tubules and increase the pregnancy rate of mice.