In order to explore influence of"Kidney Peace Granules"on death of
mesangium cell and the related ICE and Bcl 2 genetic
expression and to explain the therapeutic mechanism of nephropathy due to mesangial proliferation,35 rats were randomly divided into the control group(Group A),high dose group of Chinese medicine(Group B),medium dose group of Chinese medicine(Group C),low dose group of Chinese medicine(Group D)and cytoxan(TCX) group(Group E),7 rats each group,and were respectively fed physiological saline(Group A),"
Kidney Peace Granules"(Group B,C and D)and CTX,for respective management of serum after 8 days,by external experimental study of
mesangium cell culture,to determine the proliferation indexes of the mesangium cell and observe the dying cell and breaking phenomenon of nucleosome DNA and determine ICE and bcl 2 changes.The results showed that in serum 16 hours after"Kidney Peace Granules"was used,the typical dying sign of mesangium cell appeared,with the dying rate remarkably higher than Group A( P < 0.01 )and ICE expression obviously higher in Group B,C and D than in Group A( P < 0.01 )and without obvious bcl 2 genetic expression in various groups( P > 0.05 ).These findings indicate that"Kidney Peace Granules"can induce the death of mesangium cell,promote dispersion of the proliferating change of the glomerulus and may influence the death by regulating ICE and bcl 2genes.