Objective To investigate the protective effect of
N methyl glucamine(N MG)on Cisplatin(CDDP) induced nephrotoxicity
in rats and its possible mechanism.Methods Male Sprague Dauley rats were injected i.p.with 5 mg/kg CDDP combined with 2 g/kg N MG given i.g 1 hour before the adminstration of CDDP.BUN,Urinary protein and platinum contents were determined.Kidneys were analyzed for NOS,NO,MDA,SOD and platinum on 3,5 and 7 days after treatment.Moreover,the effect of N MG on the antineoplastic activity of CDDP was measured in mice bearing S 180 .Results N MG alleviated nephrotoxicity induced by cisplatin on day 3 and 5 after administration of CDDP.At the same time,NOS,NO and MDA contents decreased and SOD activities restored ( P<0 05 or P<0 01 ).The excretion evels of urinary platinum increased significantly on day 3 after treatment( P< 0 01),while renal platinum contents decreased on both day 3 and 5.The combination of CDDP and N MG didn't influence the inlibition tumor,action of CDDP.Conclusion These results indicated that N MG was useful for prevention of renal damage induced by CDDP.N MG mediated protection might be related to a reduced exposure of the renal tissue to platinum during the course of its enhanced excretion.As a result,free radicals induced by CDDP were decreased and the extent of
lipid peroxidation was ameliorated in rat kidneys.Moreover,the combination of CDDP and N MG would not interfere with the antitumor action of CDDP for S 180 sarcoma.