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Shvoong Home>Medicine & Health>Determination of sabutamol in human plasma by reversed phase HPLC with UV detection Review

Determination of sabutamol in human plasma by reversed phase HPLC with UV detection

Article Review   by:TsingHua     Original Author: CHINESE PHARMACEUTICAL JOURNAL
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OBJECTIVE This paper established a high performance liquid chromatography for the determination of salbutamol (SBA) in human plasma using morphine as the internal standard.METHOD A variable wavelength UV detector and Ultraspher Cyano column (5 μm,250 mm×4.6 mm,Beckman)were used.To the plasma sample 1.0,0.5 ml phosphate buffer (2.0 mol·L -1 ,pH9.0) containing 0.4% diphenylboric acid 2 aminoethyl ester was added,then extracted with 4.0 ml chloroform containing 1% tetraoctylammonium bromide.The organic layer was removed and extracted again with 200 μl (0.08 mol·L -1 ) acetic acid.100 μl of the acid layer was injected onto the column .The mobile phase of acetonitrile methanol pH2.8,0.025 mol·L -1 phosphate buffer (2∶0.5∶97.5) was pumped at the rate of 1.0 ml·min -1 through the column.The detector at 0.002 AUFS was set at 224 nm.RESULTS The retention times for SBA and morphine were 7.0 min and 8.3 min,respectively.The standard curve was linear over the concentration range of 0.5 to 32 μg·L -1 .The detection limit in plasma was 0.3 μg·L -1 .Extraction recovery was 93%~99%;The method recovery was 98%~109%;with intra day RSD less than 5%;inter day RSD less than 8%.CONCLUSION This method was found to be simple,rapid,sensitive and accurate for the determination of SBA in human plasma.
Published: July 15, 2000   
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