Objective Observe the reliability of the
medicinal serum method.Method Get osteoclasts from one-day-old SD rats' long bones,vaccinate
them on thin ivory slices,get out at the 7th day of culture,count the
bone absorptive lacunae in each group.Choose western medicines Fushanmei,Guban and Beimeili which can apparently inhibit the bone absorptive action of osteoclasts,make
medicinal serum by stomach-perfusion with equivalent dosages which are respectively compared with the groups direct added with corresponding medicines in three concentrations(1×10 -8 M,1×10 -6 M,1×10 -4 M),then observe the changes of bone absorptive lacunae.Under the same condition make control group with stomach-perfusion of saline and get its serum.Result The medicinal serum of three medicines and the lacunae of each group with direct addition are much lower than that of control.Among which the medicinal serum group of Fushanmei and three concentrations groups of direct addition have no marked difference(P>0 05),so do medicinal serum group of Guban and Beimeili with direct addition of 10 -8 M,but the their acting strength is lower than that of direct addition group of 10 -8 M and 10 -4 M(P<0 05 or P<0 01).Conclusion The medicinal serum method has a somewhat reliability,the pharmacological intensity of the medicinal serum made under this experimental condition roughly equals to that of direct addition in concentration 10 -8 M.