Objective:To observe the changes of arterial tension and the role of endogenous nitric oxide (NO) and prostacyclin(PGI
2)in high fat diet rats Methods:SD rats were divided into high fat diet group and normal control group After feeding 5 weeks,blood samples were collected from all of the rats for detecting serum lipoprotein,and aortas were harvested for measurement of the responses to norepinephine (NE) and acetylcholine (ACh) Result:Compared with the control group,the level of serum total cholestrol(TC),low density lipoprotein (LDL) was markedly increased in high fat diet group ( P<0 01,P <0 05,respectively) The
response of NE induced
vasoconstriction was significantly decreased,( P<0 01 or P <0 05),and of ACh induced relaxation was decreased too,but P >0 05 After incubated with indomethacin,the response of NE induced vasoconstriction in the aorta was enhanced,( P<0 01 or P <0 05),of ACh induced relaxation was not obviously changed When treated with N ω nitro L arginine (L NNA) simultaneously,further enhanced NE induced contraction and partially inhibited ACh induced relaxation were observed Conclusion:Both the ability of vasoconstriction and that of vasodilatation were reduced in high fat diet rats The inhibition of NO production can aggravate the decrease of vasodilatation,and the inhibition of PGI 2 production did not cause this response