AIM: To study the in vitro preventive effect of
Ginkgo biloba extracts (Egb761) on
myocardial ischemic reperfusive injury in SD
rats. METHODS: Thirty SD rats, which were divided into 3 groups (n 1=n 2=n 3=10), were chosen to make the model of myocardial ischemic
Reperfusive injury of SD rats by using developed langendorff methods. After 10 min of perfusion with Krebs Henseleit bicarbonate buffer (KHBB), the hearts of SD rats in the test group were arrested by the cardioplegia with Egb761, while those in the placebo group were arrested only by the cardioplegia without Egb761. Sixty min later, all the hearts of SD rats were reperfused by the KHBB, and the coronary flow, heart rate, left ventricular pressure were measured at 15, 30, 60 min after reperfusion, the perfusive fluid of the hearts were collected to measure the value of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK). The myocardia of the rats were tested by the electron microscope. RESULTS: The coronary flow decreased both in the test group and in the placebo group after the early reperfusion, but it recovered more quickly and more significantly in the test group (P<005). The values of LDH and CPK in the test group were lower than those in the placebo group (P<001,P<0001) and the changes of the
Myocardial ultramicrostructure were less significant in the test group. CONCLUSION: It is suggested that Egb761 could prevent the myocardial
Ischemic reperfusive
Injury in SD rats.
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