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Shvoong Home>Medicine & Health>Nerve growth factor prevents apoptosis induced by N methyl D aspartate and hypoxia/hypog Review

Nerve growth factor prevents apoptosis induced by N methyl D aspartate and hypoxia/hypog

Article Review   by:TsingHua     Original Author: CHINESE PHARMACOLOGICAL BULLETIN
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AIM To study protective effect of nerve growth factor(NGF) on apoptosis induced by N methyl D aspartate (NMDA) and oxygen/glucose deprivation. METHODS Primary cerebral cortical cultures from rat fetus were used to observe effects of NGF on the inhibition of apoptosis. RESULTS NMDA 30 μmol·L -1 exposured to cortical cell cultures for 20 min or oxygen/glucose deprived cultures for 10 h elicited typical apoptosis biochemical and morphological changes including condensed chromation, nuclear fragmentation, and reduction in volume and DNA degradation by endonucleases into fragments in oligonucleosomes of 180 ̄200 bases pairs. In addition, the characteristic apoptotic DNA peak fo the cells (i.e. sub G 1 peak) was revealed by flow cytometry. Apoptotic cell ratio was 50 2% and 45 7%, respectivelly. In agarose gel electrophoresis, the DNA extracted from cultured cerebral cells treated with NMDA (300 μmol·L -1 ) or hypoxia/hypoglycemia showed a typical internuclesosmal DNA degradation, i.e. DNA ladder. Previously incubating with NGF (50,100 μg·L -1 ) inhibited NMDA or hypoxia/hypoglycemia induces apoptosis of cultures rat cerebral cells. CONCLUSIONS NMDA(300 μmol·L -1 ) or hypoxia/hypoglycemia induced apoptosis in cerebral cortical cultures and NGF reduced apoptosis.
Published: February 25, 1999   
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